THE EFFECT OF ZINC OXIDE NANOPARTICLE CONCENTRATION AS A COATING FOR HEAT CURED ACRYLIC RESIN ON Candida Albicans GROWTH

Abstract

The most commonly used polymer material as a denture base is heat cured acrylic resin because it has high strength, accuracy, and aesthetics. However, heat cured acrylic resin has a disadvantage in biological properties, namely it does not have antimicrobial properties. This study aims to test the effect of ZnO nanoparticle concentration as a heat-cured acrylic resin coating on the growth of C. albicans. 24 acrylic resin samples were required in this study, measuring 13mm x 13mm x 2mm. The acrylic resin was coated with 3-(trimethoxysilyl)propyl methacrylate) and then coated with ZnO nanoparticles with varying concentrations of 2.5%, 5%, and 7.5% in ethanol. The ZnO nanoparticle coating was applied using the dip coating method. Samples were made in groups I, II, III, and IV. The samples were then tested for C. albicans growth. The data obtained were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test. The results showed that the mean and standard deviation of C. albicans growth after contact with acrylic resin coated with ZnO nanoparticles in groups I to IV were 2.00±0.89; 0.83±0.75; 0.00±0.00; 0.00±0.00. The Kruskal-Wallis test results showed a significance value of 0.002, which means that the concentration of ZnO nanoparticles as a coating had a significant effect on the growth of C. albicans on heat cured acrylic resin (p<0.05). The conclusion of this study is that the addition of zinc oxide nanoparticles as a coating on acrylic resin can reduce the growth of C. albicans statistically significantly.