ASSOCIATION OF CDH1 -160 C/A (rs16260) POLYMORPHISM WITH METASTASIS IN BREAST CANCER

Ida Bagus Gede Ari Suryawan; I Wayan Surudarma; I Gede Putu Supadmanaba; Ni Putu Tesi Maratni; Putu Anda Tusta Adiputra

Authors

Ida Bagus Gede Ari Suryawan Program Studi Sarjana Kedokteran dan Profesi Dokter, Fakultas Kedokteran, Universitas Udayana Author
Wayan Surudarma Author
I Gede Putu Supadmanaba Author
Ni Putu Tesi Maratni Author
Putu Anda Tusta Adiputra Author

Keywords:

Breast Cancer, Metastasis, CDH1, Polymorphism, rs16260

Abstract

Metastasis constitutes the primary cause of mortality in breast cancer, driven by the loss of intercellular adhesion and the activation of the Epithelial-to-Mesenchymal Transition (EMT). The CDH1 gene encodes E-cadherin, a critical tumor suppressor. The -160 C/A (rs16260) single nucleotide polymorphism (SNP) in the CDH1 promoter suppresses transcriptional activity, potentially influencing metastatic progression. However, data regarding this polymorphism in the specific patient population at Prof. dr. I.G.N.G. Ngoerah General Hospital remains unexplored. This study aimed to investigate the association between the CDH1 -160 C/A polymorphism and distant metastasis in breast cancer patients at Prof. dr. I.G.N.G. Ngoerah General Hospital. An observational analytic cross-sectional study was conducted using genomic DNA from 22 breast cancer patients, with genotyping performed via PCR and Sanger sequencing. Metastasis status (M0 vs. M1) was correlated with genotypes (Wild Type [CC] vs. Mutant [CA/AA]) using Fisher’s Exact Test and Odds Ratios (OR). The results indicated that the mutant genotypes (CA/AA) in this study population was high (86.4%). Of the 22 subjects, 4 (18.2%) had metastasis (M1). Statistical analysis showed no significant association between the CDH1 -160 C/A polymorphism genotype and metastasis status (Fisher's Exact Test, p-value = 0.073). In conclusion, no significant association was found between the CDH1 -160 C/A (rs16260) polymorphism and metastasis in breast cancer patients at Prof. dr. I.G.N.G. Ngoerah General Hospital.

Keywords: Breast Cancer, Metastasis, CDH1, Polymorphism, rs16260.

Author Biography

Wayan Surudarma

Dr. dr. I Wayan Surudarma adalah dosen Biokimi di Fakultas Kedokteran, Universitas Udayana. Beliau mengajar mata kuliah biokimia dasar hingga klinis dengan pendekatan molekuler–klinis.

Bidang riset yang diminati meliputi metabolisme penyakit, stres oksidatif, biomarker molekuler, dan pengaruh nutrisi serta fitokimia lokal terhadap jalur biokimia. Beliau aktif dalam publikasi ilmiah, kolaborasi riset, pembinaan mahasiswa, reviewer jurnal, serta pengembangan kurikulum berbasis biomedik.

Dr. Wayan berkomitmen pada peningkatan pendidikan kedokteran berbasis ilmu molekuler dan pengembangan penelitian yang relevan bagi kesehatan masyarakat, khususnya di Bali.