ASSOCIATION BETWEEN THE RS2280091 POLYMORPHISM IN THE ADAM33 GENE AND CHILDHOOD ASTHMA

Abstract

Background:Asthma is a non-communicable disease that remains a global health concern, influenced by both environmental and genetic factors. The disintegrin and metalloproteinase 33 (ADAM33) gene has been identified as a susceptibility gene for asthma. This study aimed to investigate the association between the rs2280091 polymorphism in the ADAM33 gene and the risk of asthma in Indonesian children.

Methods:A total of 60 buccal swab samples were collected from children aged 4–18 years, consisting of 13 asthma cases and 47 healthy controls. Genotyping of rs2280091 was performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis.

Results:The AA and GG genotypes were more frequent among asthma cases (69.23% and 7.69%, respectively) compared to controls (63.83% and 6.38%). Similarly, the A allele was detected more frequently in asthma cases (80.77%) than in controls (78.72%). However, no significant association was observed between the rs2280091 polymorphism and asthma risk (P = 1.0, OR [95% CI] = 0.8 [0.0779–8.592]). In addition, asthma was more commonly observed in boys (84.62%) than in girls (15.38%).

Conclusion:This study did not find a significant association between the rs2280091 polymorphism in the ADAM33 gene and childhood asthma. Further research involving larger sample sizes and additional ADAM33 polymorphisms is warranted to better understand the genetic susceptibility to asthma in children.