INHIBITORY EFFECTIVENESS OF WHITE TURMERIC (Curcuma zedoira) EXTRACT AGAINST Eschericia coli ATCC 25922

Conny Bella Septya; Komang Januartha Putra Pinatih; Agus Eka Darwinata; Ni Made Adi Tarini

Authors

Conny Bella Septya Program Studi Sarjana Kedokteran dan Profesi Dokter, Fakultas Kedokteran, Universitas Udayana Author
Prof. Dr. dr. Komang Januartha Putra Pinatih, M.Kes Translator
dr. Agus Eka Darwinata, S.Ked., Ph.D Translator
Dr. dr. Ni Made Adi Tarini, S.Ked, Sp.MK (K) Translator

Keywords:

Curcuma zedoaria, Escherichia coli, inhibition zone, antibacterial, disk diffusion

Abstract

The use of natural ingredients as antibacterial agents has been increasingly studied as an
alternative in infection treatment. White turmeric (Curcuma zedoaria) contains active compounds
such as curcuminoids, flavonoids, and essential oils, which are presumed to have antibacterial
potential. However, its effectiveness against Escherichia coli ATCC 25922 has not been widely
proven scientifically. This study aimed to determine the effectiveness of white turmeric extract
against the growth of E. coli. This true experimental study with a post-test only control group
design used Escherichia coli ATCC 25922 isolates divided into five groups: negative control
(DMSO), positive control (ciprofloxacin), and white turmeric extract at concentrations of 50%,
70%, and 90%. The antibacterial test was carried out using the disk diffusion method on Mueller
Hinton Agar, with five replications. The inhibition zones were measured after incubation for 18–
24 hours at 37°C, and the data were analyzed using the Kruskal–Wallis test. Observations showed
that inhibition zones appeared only in the positive control group (ciprofloxacin), with a mean
diameter of 32.4 mm. The negative control (DMSO) and all white turmeric extract groups (50%,
70%, 90%) showed no inhibition zones (0 mm). White turmeric extract has not demonstrated
effectiveness against the growth of Escherichia coli ATCC 25922. Further research using different
methods or concetrations is recommended.

Author Biographies

dr. Agus Eka Darwinata, S.Ked., Ph.D

Lecturer in Microbiology Department, Faculty of Medicine, Udayana University.
Dr. dr. Ni Made Adi Tarini, S.Ked, Sp.MK (K)

Lecturer in the Faculty of Medicine, Udayana University. Born in Denpasar, Bali on May 14, 1976. Has completed elementary school to senior high school in Denpasar namely SD 1 Saraswati Denpasar, SMPN 10 Denpasar, and SMAN 1 Denpasar (1982 - 1994). Medical education was completed at the Faculty of Medicine at Udayana University in 2000. Continuing studies in the Clinical Microbiology Specialist education program at the University of Indonesia in 2005 and obtaining a clinical microbiology specialist degree in 2009. A Doctorate Degree in biomedical medicine at Udayana University was obtained in 2020. currently active as a lecturer in the clinical microbiology specialist medical education study program at the faculty of medicine, Udayana University. Conducting research in the fields of bacteriology, antimicrobial-resistant and infection control.