THE EFFECT OF LEMON (CITRUS LIMON) ETHANOL EXTRACT CREAM ON COLLAGEN DENSITY IN MALE WISTAR RATS (RATTUS NORVEGICUS) AFTER EXPOSURE TO ULTRAVIOLET RAYS

Dewa Ayu Adistya Paramitha; Ida Ayu Ika Wahyuniari; Ni Made Linawati; I G Kamasan Nyoman Arijana

Penulis

Dewa Ayu Adistya Paramitha Program Studi Sarjana Kedokteran dan Profesi Dokter, Fakultas Kedokteran, Universitas Udayana Penulis
Ida Ayu Ika Wahyuniari Penerjemah
Ni Made Linawati Penerjemah
I G Kamasan Nyoman Arijana Penerjemah

Kata Kunci:

Citrus limon, kepadatan kolagen, penuaan dini akibat sinar matahari, ultraviolet B, antioksidan.

Abstrak

Background: Exposure to ultraviolet B (UVB) radiation causes premature aging characterized by collagen fiber degradation due to the formation of Reactive Oxygen Species (ROS). Lemon (Citrus limon) contains high levels of vitamin C and flavonoids, which have antioxidant properties and therefore have the potential to inhibit collagen degradation.

Objective: This study aimed to evaluate the effect of lemon ethanol extract cream on collagen density in the skin of male Wistar rats (Rattus norvegicus) exposed to UVB radiation.

Methods: An experimental study with a post-test only control group design was conducted on 28 male Wistar rats. The animals were divided into four groups: Control (K, base cream + UVB), and three treatment groups receiving lemon ethanol extract cream at concentrations of 6% (P1), 10% (P2), and 14% (P3). All groups were exposed to UVB (840 mJ/cm²) three times a week for four weeks. Collagen density was measured histologically using ImageJ software. Data were analyzed using One-Way ANOVA.

Results: Histological images showed a trend of increased collagen density in the treatment group compared to the control group. The highest average collagen density was observed in group P2 (10% concentration). However, statistical analysis showed no significant difference in collagen density between groups (p > 0.05).

Conclusion: Application of lemon ethanol extract cream at doses of 6%, 10%, and 14% did not inhibit UVB-induced collagen degradation in this experimental model.