Identification Coagulase Negative Staphylococci’s Species and Antibiotic Sensitivity Isolates from Cow Milk with Subclinical Mastitis

Abstract

Coagulase-negative Staphylococci (CoNS) were bacteria that had initially been considered harmless but were identified as pathogenic in some cases. Recently, CoNS became the most commonly isolated bacteria from cow's milk, especially in cows with subclinical mastitis. CoNS species have been the most frequently identified bacteria over the past several decades, exhibiting diverse characteristics that may impact antimicrobial resistance profiles. This study aimed to identify CoNS bacterial species derived from preserved milk isolates from subclinical mastitis in cow and their sensitivity patterns to several antibiotics. Species identification was done with a phenotypic approach using the VITEK-2 compact tool. The species identification results found Staphylococcus sciuri, Staphylococcus saprophyticus, Staphylococcus cohnii, Staphylococcus vitulinus, Staphylococcus arlettae, and Staphylococcus xylosus. Antibiotic resistant tests showed that 31.25% of isolates were resistant. Penicillin G is not recommended for treating mastitis infections caused by CoNS.